Analysis Note

ControlMouse brain tissue lysate

Application

Research Sub CategoryDevelopmental NeuroscienceNeuroregenerative Medicine

Immunofluorescence Analysis: 5 µg/mL from a representative lot detected FEZ2 in human brain tissue.

Research CategoryNeuroscienceNeuroscience

Detect the FEZ2 protein using this Anti-FEZ2 validated for use in WB & IF.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Fasciculation and elongation protein zeta-2 (FEZ2) is a homolog to the mammalian FEZ1, itself an ortholog of the C. elegans UNC-76. In contrast to FEZ1, FEZ2 mRNA is widely expressed in mouse tissues. FEZ2 interacts with protein kinase C (PKC)-zeta substrate and induces neurite extension of PC12 cells when co-expressed with a constitutively active form of PKC-zeta, suggesting FEZ2 may play an important role in the morphological changes of various cells by associating with PKC-zeta in a tissue non-specific manner. FEZ2 can interact with FEZ1 through its c-terminal regions and especially its coiled-coil region. At least two isoforms of FEZ2 are known to exist.

Immunogen

KLH-conjugated linear peptide corresponding to human FEZ2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FEZ2 in 15 µg of mouse brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed